"GAS Mutants: Fueling the Evolution of Cytosine Deaminase for Cancer Gene Therapy "
Margaret E. Black, Ph.D.
Associate Professor,
Departement of Pharmaceutical Sciences
Washington State University

C. L. Willmon, D. Sussman and M. E. Black. The Role of Herpes Simplex Virus-1 Thymidine Kinase Alanine 168 in Substrate Specificity. Submitted.

S. D. Mahan, G. C. Ireton, C. Knoeber, B. L. Stoddard and M. E. Black. (2004) Random Mutagenesis of E. coli Cytosine Deaminase for Cancer Gene Therapy. Protein Engineering, Design and Selection, in press.

S. D. Mahan, G. C. Ireton, B. L. Stoddard and M. E. Black. (2004) Alanine Scanning Mutagenesis Reveals a Cytosine Deaminase Mutant with Altered Substrate Preference. Biochemistry, 43:8957-8964.

J.-E. Kurtz and M. E. Black. (2003) Enhancement of Suicide Gene Prodrug Activation by Random Mutagenesis. Suicide Gene Therapy: Methods and Reviews. C. J. Springer, Ed. Methods in Molecular Medicine Series, Humana Press, New Jersey, 90:331-344.

T. S. Stolwothy, E. Krabbenhoft and M. E. Black. (2003) A Novel Escherichia coli Strain Allows Functional Analysis of Guanylate Kinase Drug Resistance and Sensitivity. Analytical Biochem., 322:40-47.

G. C. Ireton, M. E. Black and B. L. Stoddard. (2003) The 1.14Å Resolution of Yeast Cytosine Deaminase: Parallel Divergent Evolution of Nucleotide Salvage Enzymes and Implications for Anticancer Gene Therapy. Structure, 11:961-972.

R. Wiewrodt, K. Amin, M. Kiefer, V. P. Jovanovic, V. Kapoor, S. Force, M. Chang, M. Lanuti, M. E. Black, L. R. Kaiser and S. M. Albelda. (2003) Adenovirus-Mediated Gene Transfer of Enhance Herpes Simplex Virus Thymidine Kinase Mutants Improves Prodrug-Mediated Tumor Cell Killing. Cancer Gene Therapy, 10:353-364.

H. Kubo, T. A. Gardner, Y. Wada, K. S. Koeneman, A. Gotoh, L. Yang, C. Kao, S. D. Lim, M. B. Amin, H. Yang, M. E. Black, S. Matsubara, M. Nakagawa, J. Y. Gillenwater, H. E. Zhau and L. W. K. Chung. (2003) Phase I Dose Escalation Clinical Trial of Adenovirus Vector Carrying Osteocalcin Promoter-Driven Herpes Simplex Virus Thymidine Kinase in Localized and Metastatic Hormone-Refractory Prostate Cancer. Human Gene Therapy, 14:227-241.

M. S. Kokoris and M. E. Black. (2002) Characterization of Herpes Simplex Virus Type 1 Thymidine Kinase Mutants Engineered for Improved Ganciclovir or Acyclovir Activity. Protein Science, 11:2267-2272.

W. Qasim, A. J. Thrasher, J. Buddle, C. Kinnon, M. E. Black and H. B. Gaspar. (2002) T-Cell Transduction and Suicide With an Enhanced Mutant Thymidine Kinase. Gene Therapy, 9(12):824-827.